Improved Recovery of a Radlolabeled Peptide with anAlbumin-Treated Reversed-Phase HPLC Column

improved Recovery of a Radlolabeled Peptide with an Albumin - Treated Reversed - Phase HPLC

The results in Figure la were obtained with a column that had already been used in >300 protein and peptide purifications. When we replaced this with a new column from the same manufacturer, there was a dramatic decrease in recovery of the labeled peptide (Figure lb), as shown by the absence of the 1-34 PTHrP peak at 29 mm. Such behavior might be caused by strong or irreversible binding of pept...

Improved recovery of a radiolabeled peptide with an albumin-treated reversed-phase HPLC column.

A Reversed Phase High Performance Liquid Chromatographic Method for Determination of Rapamycin

Easily degradating and various isomeric forms of rapamycin (Sirolimus) face the determination of this compound to many challenges. In this study, we developed and validated the isocratic reversed phase high performance liquid chromatographic (RP-HPLC) method for rapamycin. Separation was performed on a C8 column (MZ, 15 × 4.6 mm, 5 µm particle size) using methanol:water (80:20 v/v) as the mobil...

A Reversed Phase High Performance Liquid Chromatographic Method for Determination of Rapamycin

Easily degradating and various isomeric forms of rapamycin (Sirolimus) face the determination of this compound to many challenges. In this study, we developed and validated the isocratic reversed phase high performance liquid chromatographic (RP-HPLC) method for rapamycin. Separation was performed on a C8 column (MZ, 15 × 4.6 mm, 5 µm particle size) using methanol:water (80:20 v/v) as the mobil...

Application of Narrow-Bore HPLC Columns in Rapid Determination of Sildenafil Citrate in Its Pharmaceutical Dosage Forms

     A special type of silica-based columns has been recently introduced into the market which is called narrow-bore columns. They have lower internal volume than the standard high-performance liquid chromatography (HPLC) columns and thus reduce the solvent consumption by almost 80%.    A simple, accurate and environmentally friendly reversed phase- HPLC (RP-HPLC method) which could be used in ...